Artificial Oocyte Activation is a modern specialized IVF laboratory technique that assists in triggering egg activation, improving fertilization in cases where fertility is not progressing as expected.

For couples who have experienced failed or poor fertilization, Artificial Oocyte Activation (AOA) represents a promising and innovative technique designed to improve reproductive outcomes, providing the necessary boost, activating the ovarium.

At Crete Fertility Centre, we continuously adopt innovative fertility treatments and laboratory technologies that maximize each patient’s chances of achieving a successful pregnancy. Artificial Oocyte Activation is one of the specialized techniques offered in selected cases of recurrent fertilization failure.

Benefits of Artificial Oocyte Activation

Artificial Oocyte Activation procedure carried out in Crete Fertility Centre provides significant benefits for the candidate couples.

• Offers a solution to repeated fertility failures.
• Significantly increases success rates in difficult cases.
• Brings new hope to couples which have been disappointed by previous attempts
• It can prove to be decisive for the positive development of an IVF cycle.

Moreover, this technique is applied as a part of a holistic approach of personalized care. Having in mind that each couple is unique, Artificial Oocyte Activation (AOA) is applied only when it is truly indicated, based on your own data and needs. The vast experience of our medical team alongside with its high expertise outcomes the use of this innovative scientific method, turning the possibilities of a successful fertilization into prospects.

How Artificial Ovary Activation (AOA) is applied

AOA can be performed in different ways, depending on the needs of each case:

After microfertilization (ICSI), the eggs are placed for a few minutes in a special culture medium containing calcium ionophores (inomycin).

Calcium ionophores are substances that help calcium enter the interior of the egg. In this way, they mimic the natural “signal” given by the sperm, activating the egg to begin embryo development.

They help initiate reactions necessary for embryo development, increasing the percentage of blastocysts.

When is AOA recommended

The technique can provide a solution in cases such as:

• Total fertilization failure after ICSI
• Low fertilization rates (below 33%)
• Severe male factor infertility (low quality sperm),
• Oocyte activation deficiency, when the egg itself does not respond normally

Effectiveness & Safety

Artificial Oocyte Activation is a promising technique that:

• Significantly increases fertilization rates
• Improves the qualitative development of embryos (e.g. blastocyst formation)
• Enhances the chances of pregnancy, especially in couples with previous failed attempts

It is implemented with strict laboratory protocols, based on the available scientific data on its safety and effectiveness.

Give fertilization the chance it deserves

Crete Fertility Centre is an IVF destination for every couple seeks for successful results in its journey to parenthood. The application of Artificial Oocyte Activation in our centre is another proof for our high expertise and innovative techniques we offer. This unique combination of innovation and patient care in Crete Fertility Centre is a major reason why we bring hope and positive results to whoever trust us.